Engineer wanted for HCS and Super Resolution Microscopy

Engineer in computer science for microscopy at the Interdisciplinary Institute for Neuroscience (IINS, UMR 5297CNRS), Bordeaux

Team: Quantitative Imaging of the Cell

Type of contract: 12 months, renewable, full time

B.S. /M.S. in a computer science, bioinformatics or biotechnology with 1 to 3 years of work experience

Salary: commensurate with experience

Characteristic duties

We are searching a motivated engineer to create and expand on existing software solutions dedicated to biologists’ needs

for the acquisition and analysis of High Content Screening (HCS) experiments on super-resolution optical microscopes.

Under the responsibility of the project leader and in close collaboration with the biology and bioinformatics teams, he/she

will:

  • Develop the software and graphical user interface for the acquisition (screening) and create a database from

super-resolution microscopic image analysis (localizations, tracking, …)

  • Implement new classification methods and evaluate them on biological data.

Requirements

  • Language: Visual Basic .NET, C/C++, Python. Optional : Matlab, Java, R.

  • Database IT, SQL.

  • Knowledge of fundamentals of microscopy (Experience with Metamorph is a plus).

  • Excellent communication skills, openness, and ability to collaborate in an interdisciplinary environment.

The team

The “Quantitative Imaging of the Cell” team develops novel imaging techniques to better understand the living cell activity

at high spatial and temporal resolutions, in a high throughput context. Three main research area are investigated:

  • Novel instruments for high-resolution microscopy of living samples, focusing on the development of new

instruments for Single Molecule Tracking by Photo-Activation Localization Microscopy (SPT-PALM), Local

Photoperturbation Microscopy (FRAP/PA), 3D imaging of thick biological specimens (Multi-photon Imaging) and

Structure Illumination microscopy (SIM, Compress Sensing).

  • Analytical tools for object segmentation, tracking and visualization using CPU and GPU.

  • High Content Screening Microscopy to quantify the dynamics of active proteins within the living cells, using super-
    resolution microscopy and micropatterning/microfluidics to control cell geometry and their local chemical

environment.

The team works in close collaboration with the Bordeaux Bioinformatics Center (CBiB) and some industrial partners

Contact

Jean-Baptiste Sibarita: jean-baptiste.sibarita@u-bordeaux2.fr