Emulsion image analysis- want to find polydispersity

3 % PGPR with scale12.tif (797.6 KB)

This image is of emulsion. I have observed emulsion under microscope and taken photos with dino light.
I want to analyse the diameter of droplets.
Thank you,
Rajanikant

Hi @rajanikant_savant

Thanks for the image. Perhaps it would make sense if you could annotate what is a droplet according to you, as I see many different structures in your image.


light and dark circular spots are droplets.
Thank you,

Have you considered using Ilastik to start identifying the droplets? This could be used to do some initial detection and then imported into CellProfiler. We have a tutorial for a similar workflow here.