I heard about cell profiler at the last ASCB meeting in December. we have been looking for such a software for quite a while now and yours seems very exciting. I have a couple of questions, very basic.
we are mainly using fixed mammalian tissues for IF, IHC, H&E, …, and EM. Have you tried to use CellProfiler on such samples? We would mainly like to be able to measure cell (or organite) size.
I have tried, but have not been very successful.