I was hoping to get some clarification on the edge mean intensity. We’re trying to measure the amount of transferrin bound to the cell surface versus the intracellular pool. Up until now we’ve been reducing the cell boundary (stained by phalloidin) by a pixel and subtracting that from the cell boundary to measure the transferrin intensity “on the surface”.
I then suddenly realised that a simpler (and correct?) approach would be to simply take the edge mean intensity value for the transferrin measurement and compare it to the overall intensity of the cell? I’m unsure how the edge and overall mean intensity are related and if they can be compared directly?