Any advice on how to approach these image analysis applications? In my image attached I have a green and red stain that both saturate to black. Depending on where I set my color vectors, I tend to have to compromise detection between these two colors.
Any tips or tricks on how to approach these images? Should I try to use a different color deconvolution algorithm to separate these colors? I’m open to FIJI or Qupath workflows. Or do I need to ask the wetlab to adjust the staining parameters to “lighten” things up?
At this point, all I care about is getting total area green and total area red.
Sorry I don’t have access to a higher quality image that I can distribute.
Any advice is appreciated!