Drosophila courtship analysis



I need to do quantitative analysis of drosophila courtship behavior. Is there any Fiji/ImgaeJ based plugin or macro one can use for this. the videos are of around 10 minutes long and are around 1 GB of size. this is basically one fly following the other in an arena, and we need to find out how long out of 10 minutes the male fly is courting.

best regards


Not knowing more details about your video (you left no screenshot or a detailed description of the setup) I would guess that a tracker might identify the courting in the flying pattern, see TrackMate from @tinevez:

You find a lot of post of TrackMate in this forum already:



I have uploaded the screenshot.The other problem is, I am unable to open these videos in ImageJ/Fiji and the message I am getting is . the files are in avi format only.


Here you find some links to import video files from different formats in ImageJ:

The cause of your error is a wrong format which ImageJ can’t read so you have to convert your file.