I’m relatively new to this forum and to the image analysis at all. I have a (probably trivial) problem to submit you.
I need to measure the pixel intensity (gray values) along some specific profiles of an active selection (i.e. particle). In particular, I need to obtain the profile as a list of pixel grey values along both the Feret diameter (maximum caliper) and FeretMin (minimum caliper) of the selected particle in the reported image.
To do this, at now, I’ve selected the particle and used a macro in order to draw the Feret above the particle and to measure the intensity profile with the command ‘plot profile’, but it resulted in the complete erasure of the original pixel gray values.
So, I need to know how to identify the Feret and FeretMin of an active selection, and to record them for example as straight line selection in order to directly measure the profile using ‘plot profile’.
I provide the image below as an example: I’ve selected the shape of which I’d like to know the profile in pixel intensity along both the Feret and Feret Min.
Tanks in advance for every possible solution or even suggestions