I just started using CellProfiler yesterday and I’ve already learned a lot thanks to the tutorials and this forum.
I am trying to analyze IF cell staining with DAPI, CD45, and DsRed with the hopes of calculating single and double positives.
I think I have the pipeline working but I’m having trouble adjusting the parameters. Some of the objects look like they are more than one cell. I’m hoping you can point out what I’m doing wrong. I’m unfamiliar with the different thresholds to choose from.
12-0327 Paige.cp (20.2 KB)