@emartini Let us say, white is 1, black is 0 and the grey colours are something between 1 and 0. Now I have one picture before something happend and one picture after something has happend. So I want to devide the first picture through the second picture.
@ctrueden No, not really thresholding. I have one substance, which I have measured and an internal standard for a quantification. Maybe the discription above is good. If I have at both pictures the value 1, both substances have the same concentration and so on. I hope you might understand it better now.
@anon96376101 I will try it in the next minutes and I will keep you updated, if you are right. At first, it really sounds pretty good. Sorry, that I have not found it. Actually, I was not really show what I have to search for.
Thank you all!!!