Dear QuPath users. I am very new in QuPath and haven’t found a solution on my problem, which is why I am adressing you. Any help is very appreciated
In my lab, we stain our melanoma tumor tissue from paraffin blocks by using a pink-reddish AP-Polymer. We do this, as every now and then melanotic tumors can be quite pigmented and thus, a distinction between positively stained cells and pigmented cells would be difficult with DAB in brightfield scans.
So far, I’ve been successful to find positively red-stained cells, by thresholding the DAB intensity in non-pigmented tumor samples. But:
Is there a way, to distinguish red and brown and select only for strongly red cells in qupath? Or do you have any suggestions on alternatives?
I thank you already in advance for your help and kind regards