Distinguish overlapping spindle-like cells

cellprofiler
#1

Hi everyone!

I’m quite a newby in using CellProfiler, which is why I need your help.
I have spindle-like cells which overlap quite a lot in my images and I need CP to distinguish between them. My goal is to classify the different morphology of the cells, so the right identification is what makes or breaks the whole analysis.

The problem is, that CP does distinguish partially, but not in the way of the cells morphology.
Here’s a example picture:

I’ll add you my pipeline for what I have done so far: OverlappingCells.cppipe (14.1 KB)

Thanks in advance!

BR,
Leonie