I’m in a middle of a project and I need your help, if possible,
I’m desperate to count nuclear immuno-histochemical positve cells using as a chromogen magenta red. I used this chromogen because the lungs I’m examining are full of hemosiderin (brown pigment).
Using positive cell counting ,both with hematoxylin OD or OD sum it count as positive both hemosiderin pigments and these cells. Playing with the images filter I understand why both struggle to recognise them, although using OD sum defines them as definetly separate entities.
Any help would be much apprecciated
Thanks a lot