I wanted to quantify macrophages using CD68 marker. As proposed by Mike and Pete, I’ve quantified positive pixel counts in all my slides. However, I observed that 30% of tumors have a lot a stroma in which few tumor nests are surrounded by many macrophages (like clusters) whereas the others carry big tumor nests surrounded by little stroma containing macrophages.
My question is:
would you think it relevant
- to quantify peri-tumoral macrophages ?
- normalizing macrophages quantity by stroma quantity (by using a stromal marker)?
If I go for peri-tumoral macrophages quantification, how to define the peri-tumoral area (a distance between tumoral cells at the surrounding and X µm of stroma) ? Is it possible with qupath ?
Thanks for your help !!