I’m new to Fiji and to this forum, which has already helped me a lot, but I’m still having huge troubles to analyze my images…
Here is what I’d like to do: I’d like to analyze the localization and distribution of the green-positive cells regarding to the red-positive regions. The tissue labelling was quite challenging so I have to define the red-regions and the green-positive cells manually.
Picture1-roi.tif (1.9 MB)
I was thinking of defining a line centered to my red regions (as they have an unusual form, I cannot use the centroid point, but is it possible to calculate the equivalent of a ‘centroid line’ or ‘centroid shape’?). Then run an Euclidean distance map originating from those lines/shapes and finally measure the distance of each green-positive cells from a red center thanks to this map.
I’d also like to have a distribution of the distances between my green-positive cells to possibly evidence clustering.
Could you help me achieve that?
Thanks a lot in advance,
NB: the picture quality is very bad, sorry, I’m currenty homeworking and don’t have access to the original images…