I’m pretty new to image analysis so this might seem a minor problem. I have a .tif animation of 4 fluctuating points (994 frames), and I need to measure distances between those points for each frame. Is there a plugin or a macro that can automate such a task?
Thanks in advance.
For an example workflow using KNIME to process the TrackMate output data, see the supplementary material of this publication (Disclaimer: I am a co-author):
Hauer et al.
Histone degradation in response to DNA damage enhances chromatin dynamics and recombination rates
Nature Structural & Molecular Biology 24, 99–107 (2017)
Thank you! Worked like a charm.