Distance between cell types and area of interest

Hi all,

I am new to ImageJ but I try to learn more about it.

Here is the challenge I am facing right now:
I am counting cell types which are triple positive for my markers of interest on my fluorescent image. Those cells are in the liver and I want to know if they are randomly distributed in my tissue, or if they are close to some area of interest (e.g. portal field, veins). I would also like to know the same information about the single positive for one of the fluorescent marker.
Automating the measurements would be of course amazing but I do not know if this is doable using ImageJ and its plugins only.
To summarize, I have cells A (triple positive) and cells B (single positive) and I want to know if they are randomly distributed and they are not clustering around an area C.

Thanks in advance for giving me tips about how to do that.
Best,
Maxime

This thread discusses a related question using R:


Jerome.

Thank you Jerome, I will have a look at the thread and see if it is doable that way. :wink: