I am new to ImageJ but I try to learn more about it.
Here is the challenge I am facing right now:
I am counting cell types which are triple positive for my markers of interest on my fluorescent image. Those cells are in the liver and I want to know if they are randomly distributed in my tissue, or if they are close to some area of interest (e.g. portal field, veins). I would also like to know the same information about the single positive for one of the fluorescent marker.
Automating the measurements would be of course amazing but I do not know if this is doable using ImageJ and its plugins only.
To summarize, I have cells A (triple positive) and cells B (single positive) and I want to know if they are randomly distributed and they are not clustering around an area C.
Thanks in advance for giving me tips about how to do that.