I’ve been successfully processing multi-timepoint tiff files produced by Incunosys Zoom system in CellProfiler. It recognizes tiff-files’ multiple timepoints and I get out very nice data.
However when I try to process results in CellProfiler Analyst I ran into following issue.
It seems that opening images for classification or in image viewing produces following error
An error occurred in the program:
JavaException: For input string: “1,219998”
Traceback (most recent call last):
File “cpa.py”, line 287, in launch_image_viewer
File “imageviewer.pyc”, line 227, in init
File “imageviewer.pyc”, line 675, in OnOpenImage
File “imagetools.pyc”, line 52, in FetchImage
File “imagereader.pyc”, line 29, in ReadImages
File “imagereader.pyc”, line 46, in read_images_via_cp
File “bioformats\formatreader.pyc”, line 470, in load_using_bioformats
File “cellprofiler\utilities\jutil.pyc”, line 308, in run_in_main_thread
Does this mean that tiff-format that I’m using is not supported or do I need to tweak some settings in .properties file? I already tried image_size_info = Y and N but it did not help in this.
Is a good “plan-b” to this that I somehow split the tiff files to individual frames and then name of the frames can be found from database CPA uses?
University of Oulu