I’m running a pipeline that identifies E. coli colonies within spots plated on square dishes. IdentifyPrimaryObjects is used to identify the spots, then again used to ID the colonies, and RelateObjects is used to associate the colonies with the parent spots. I have DisplayDataOnImage set to overlay the number of colonies in each spot on a black background. IdentifyPrimaryObjects is working correctly, but I’m getting no output from DisplayDataOnImage.
If you’re still having trouble, would you mind posting your pipeline here and we’ll take a closer look?