I’m using Fiji (ImageJ 2.0.0-rc-69/1.52n 64-bit) on Windows 10 64-bit - although I expect this isn’t version-specific. More likely this is a misunderstanding on my part about how some things work behind the scenes.
I’m processing 32-bit float TIFF stacks using 3D projection (MIP), and noticed some inconsistent results. I see that the output of that process is 8-bit, and have narrowed down the source of my problems to that conversion. Playing around with the conversion from 32-bit float to 8-bit, I noticed the following: if I adjust the image display via Image>Adjust>Brightness/Contrast - using the auto setting, but NOT applying the change - it affects the subsequent conversion to 8-bit.
For example, without Brightness/Contrast adjustment:
and with Brightness/Contrast adjustment:
The histogram of the original image doesn’t change, which is the behavior I expected. However, the histogram of the resultant 8-bit image does seem affected.
I dug through the code a bit but figured it might be faster to just ask here! Thanks in advance!