I have been trying to stitch some bright-filed images using “Grid/Collection stitcing” in FIJI. In this plugin I have used “Positions from file” and the order is “Defined by TileConfiguration”. The tile configuration values are directly extracted from image metadata. Seems this method is working fine for a small group of images ( < 100Mb fused image) but not big grids of images. If I don’t check the “compute overlap” checkbox, the the image tiles won’t be in exactly correct place and if I use the “compute overlap” some of the tiles go missing and some would be displaced. Would you please give me some advice on this issue?