I am new at using CellProfiler. Let me start by saying that I think it is pretty easy to work with and has a nice interface and informative help-functions. Unfortunately I am running into some novice problems.
I am trying to quantitate the Nuclear/Cytoplasmic distribution of a GFP-tagged protein, which I transfect into cells. So some cells will be GFP-positive, whereas others are GFP-negative.
I would like to filter out the GFP-negative cells, but so far I have been unable to come up with the right solution.
The pipeline files I have composed from scratch is at follows:
Pixel Size: 1
Could you let me know of a solution to only define cytoplasm in the GFP-positive cells? I have tried some filter and exclude modules, but so far I’ve been unable to come up with anything that works.
A confocal image illustrating the GFP and nuclear signals is posted at:
and screen dumps that hopefully illustrate the problem are found at:
Thanks a lot,