Discrepancy between macro auto-threshold versus manual auto-threshold

Dear all,

I have an image stack that I would like to apply basic global thresholding via Otsu technique.

By conducting manual steps, I have recorded a macro. However, when I apply this macro to the image stack, you can see that the results are different (attached images). I am not sure if this is a bug or me failing to create a proper macro…

Many thanks for your help.

Kind regards.

To help you, at best we would need:

  • an original image
  • your macro so that we can test.