Discard Cells that touch each-other

Hi all,

I am a beginner to CellProfiler, I have just begun by attempting to analyse cell shape of individual cells. However, I cannot work out how to disregard cells that have touching cell borders such as the ones in the image.

Any help would be much appreciated!
Alex

Hi @Researchassistant,

Welcome to the forum.

I think the way I would do this is to use a “MeasureObjectNeighbours” with the method set to adjacent and then use a “FilterObjects” to filter based on number of neighbours to be left with cells with no neighbours (so filter with a maximum measurement value of 0).

Best,
Laura

Perfect! thank you so much!