Disappearing particle -bug?

The attached image after

run("Auto Local Threshold", "method=Otsu radius=15 parameter_1=0 parameter_2=0 white");
run("Linear Kuwahara", "number_of_angles=30 line_length=11 criterion=Variance");
setOption("BlackBackground", false);
run("Convert to Mask");
run("Fill Holes");

loses a particle in the center.

Is this a bug?

Hi Andras,
You haven’t attached an image, so it is difficult to answer your question. However, could it be that the central particle is located within the hole of another particle?

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