Disappearing particle -bug?

Usage & Issues
, ,
#1

The attached image after

run("Auto Local Threshold", "method=Otsu radius=15 parameter_1=0 parameter_2=0 white");
run("Linear Kuwahara", "number_of_angles=30 line_length=11 criterion=Variance");
setOption("BlackBackground", false);
run("Convert to Mask");
run("Fill Holes");

loses a particle in the center.

Is this a bug?

#2

Hi Andras,
You haven’t attached an image, so it is difficult to answer your question. However, could it be that the central particle is located within the hole of another particle?
Regards,
Volko

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