I’m trying to segment and measure cells by relying on a tight junction staining. The staining in not optimal and there is quite some background. However, visually, it is well seen.
I’ve been trying to use different bluring and convolutions as well as the “dilate” and “erode” options to obtain a continuous mask around my cells, but this has proven difficult.
Would anybody know some alternative options and/or trick to obtain a contiuous ROI around cells when the staining is not optimal?
many thanks for your help,
Segmentation.tif (1.8 MB)