Dear all,

I was wondering if anyone had experience and or a script for the use of Image J in the assessment of myogenic differentiation index or fusion index. Differentiation index is defined as the number of nuclei in myotubes expressing myosin heavy chain divided by the total number of nuclei in a field. The fusion index is defined as the number of myosin heavy chain expressing myotubes with greater than 2 nuclei divided by the total number of nuclei.

Thank you so much

Sincerely

Mari