Different SPIM Stages and X-SPIM Configuration

openspim

#1

Greetings friends,

We are building a SPIM set up and had a few questions if anyone can kindly answer:

1.) We are developing a SPIM system and are trying to follow many elements of this system here:

https://openspim.org/SPIM

This SPIM system on the OpenSPIM website is different from the OpenSPIM parts list/configuration. The main 2 things we want to take away from this design are:

1.) Use of PI/Thorlabs XYZ/Rotation stages instead of the Picard Stage (we already have the PI 111.1DG Stages)

2.) Use of an environmental chamber as shown in the design.

The questions we had were:

1.) What is the history of that SPIM subpage and parts list within the OpenSPIM website (https://openspim.org/SPIM). Again, this is different than the OpenSPIM Table of Parts parts page: (https://openspim.org/Table_of_parts)? Is this a more advanced configuration? It seems like the edits were made by “Weber (assuming this is Michael Weber?)”

2.) If we were to use PI/Thorlabs XYZ/Rotation stages (respectively) as shown in the SPIM page I linked, can we still use the OpenSPIM plugin for acquisition? If not, does anyone know of the best way to program acquisition? LabView?

3.) Many folks have been talking about an X-SPIM configuration. In fact, the “official maker” of OpenSPIM Parts now has it shown on his website:


Is the dual detection with sCMOS cameras for better axial resolution? Or is it to rapidly image 2 channels at once? We would ideally like to set up the X-SPIM type configuration as we have the optics for it as well as dual sCMOS cameras that we are luckily able to use. Can someone provide some advice/insight? We would be very grateful.

Thank you all so so much in advance.


#2

Hello,
I can only comment on your second point:

2.) If we were to use PI/Thorlabs XYZ/Rotation stages (respectively) as shown in the SPIM page I linked, can we still use the OpenSPIM plugin for acquisition? If not, does anyone know of the best way to program acquisition? LabView?

We have sucessfully implemented the use of a Thorlabs DRV001 stepper motor connected via a Thorlabs BSC201 controller on Win 7 64bit with our OpenSPIM (see here). However swapping out a picard stepper motor for the Thorlabs DRV001 stepper motor (or any other motor for that matter) causes the SPIM Acquisition plugin to fail in Micro-Manager so I now use the Stage Control and Multi-D Acquisition plugins to take images and this works fine.
You might considder adopting ClearControl, this is something that we are keen to try soon, it looks very promising but is still in it’s infancy.

Good luck and do keep is posted on your progress.