We are building a SPIM set up and had a few questions if anyone can kindly answer:
1.) We are developing a SPIM system and are trying to follow many elements of this system here:
This SPIM system on the OpenSPIM website is different from the OpenSPIM parts list/configuration. The main 2 things we want to take away from this design are:
1.) Use of PI/Thorlabs XYZ/Rotation stages instead of the Picard Stage (we already have the PI 111.1DG Stages)
2.) Use of an environmental chamber as shown in the design.
The questions we had were:
1.) What is the history of that SPIM subpage and parts list within the OpenSPIM website (https://openspim.org/SPIM). Again, this is different than the OpenSPIM Table of Parts parts page: (https://openspim.org/Table_of_parts)? Is this a more advanced configuration? It seems like the edits were made by “Weber (assuming this is Michael Weber?)”
2.) If we were to use PI/Thorlabs XYZ/Rotation stages (respectively) as shown in the SPIM page I linked, can we still use the OpenSPIM plugin for acquisition? If not, does anyone know of the best way to program acquisition? LabView?
3.) Many folks have been talking about an X-SPIM configuration. In fact, the “official maker” of OpenSPIM Parts now has it shown on his website:
Is the dual detection with sCMOS cameras for better axial resolution? Or is it to rapidly image 2 channels at once? We would ideally like to set up the X-SPIM type configuration as we have the optics for it as well as dual sCMOS cameras that we are luckily able to use. Can someone provide some advice/insight? We would be very grateful.
Thank you all so so much in advance.