I’m measuring parameters as Area, Mean, IntDen in immunofluorescent tissue sections after subtracting the background, setting the threshold to the image and using Analyze Particles. It works pretty well but I got two different measurements in the Results window and in the Summary one.
Basically, for the Analyse particles, I use 0-infinity and 0-1 circularity, without selecting any of the ‘exclude on edges’ or ‘Include holes’ options. Nonetheless, if I hit the ctrl+M buttons after running the analyze particles, I get the same Area, same Count, same %Area between Summary and Results windows but different Mean and different IntDen. The ctrl+M command gives in the Results window what it looks like the right IntDen, since it is equal to Area*Mean value, but in the Summary window, the IntDen is a value completely different and very small.
Do you know why the Mean and IntDen values are different between the Results and Summary windows? which one is the correct one?
I would really appreciate your opinion.