I have run into a problem while computing the degree of anisotropy between BoneJ 1.4.1 and 1.4.3 on a cubic section of trabecular bone. In BoneJ 1.4.1, the degree of anisotropy is on average around 0.86, whereas in BoneJ 1.4.3 it is around 0.67. I was wondering if there were any differences in the algorithm between the two versions that could explain such a difference, and which version should we trust for our degree of anisotropy?
The parameters asked for the analyses are indeed quite different between the two versions. Here is what we entered (they are the default parameters):
For BoneJ 1.4.1:
auto mode checked, single sphere unchecked.
radius 125 pixels, vectors 50,000, vector sampling 2.300, min spheres 100, max spheres 2000, tolerance 0.0050.
show plot cheched, 3D result unchecked, align to fabric tensor unchecked, record eigens unchecked.
For BoneJ 1.4.3:
Directions 2000, lines per dimension 100, sampling increment 1, recommended minimums unchecked.
Thank you in advance for your time,