When acquiring transmitted light zstacks using 2.0g on our Olympus IX83, with auto shutter on, sequence mode enabled on our ASI Z stage, and “keep shutter open” checked in MDA, the shutter opens/closes every 50 frames in each stack. Looking at the problem report it seems like the MDA is setting up the acquisition as a series of 50 frame burst acquisitions even though this is a single z stack? With sequence mode off everything behaves as expected, I’m just not sure where this odd 50 frame behavior is coming from with sequencing on. Sequencing with the stage seems to be critical for this behavior as simply acquiring 100 frames without a zstack doesn’t stop every 50 frames.
Problem Report.txt (558.3 KB)