DiameterJ realistic frequency?

Hi,

I recently started using DiameterJ to measure the diameters of these nanofibers I’m manufacturing in my lab. I can do the basic steps to where I’m getting summaries and radius values in excel sheets, but the frequencies for the radius values I’m getting seem unrealistic. For some of my images like the one I posted below, it is easy to count and I feel as though I may be doing something wrong leading to inaccurate frequency values. Help would be greatly appreciated.

  • John