Determining cortical bone total CSA and cortical bone area

Hi Michael and others,

Firstly thanks much for such a great tool as boneJ.

I have a stack of microCT images of cortical bone of mice. I wanted to calculate total CSA and cortical bone area. This is probably a very basic question, I would very appreciate any advice. I was wondering if my procedures below are correct?

For total CSA
I converting the image stack into 8-bits & thresholding into black & white. Filling the medullary area with black/ background.
Then, I used Slice Geometry to get the CSA. It gave CSA results for each one of the slice. I took the average.

For Cortical bone area
I measuring the cortical bone volume dividing (number of slices x slice thickness) .

Thanks in advance for your comments and suggestions.


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