I am trying to establish a systematic way to detect in which image of a time lapse particles deform /merge. The particles are plastic pellets, and I am aiming at determining the melting point, related to the time. The particles move due to buoyancy, and the melting can be visualized by deformation of the pellets followed by merge. Here the first and last imaged of the secuence, DSC_8425.tif (536.9 KB)
DSC_8780.tif (536.9 KB)
A video of the full secuence can be seen here Dropbox - Autoclave_meltingT_testtest.mpg - Simplify your life
While I can visualize deformation, since the pellets are not circles but ovals, I don’t find a geometrical parameter which I could use as a threshold. I welcome ideas. Otherwise I guess I need to go to the particle merge event. I have explored Track Mate with FiJi, but I have troubles in the initial particle detection stage as the particles are not circles. Are there any work-arounds?
I am now exploring Cell Profiler and the Object Tracking sample pipeline. While I’m still working on the correct particle detection, I would appreciate feedback on if I’m going in the right direction at all. Should I process the secuence backwards, to detect when the final blob splits rather than when it merges?
Thanks in advance!