Hi, I would like to analyse spheres from a SEM image where some spheres have quite low contrast. I have been experimenting with adjusting C&B, despeckle, enhance contrast and thresholding but the biggest issue is that when I threshold a part of the low contrast spheres are lost, therefore they do not get detected as spheres but only part of the rim. For the same reason “fill holes” does not help either. Ultimately I only want to detect the area/diameter of the spheres. Any advice?
Thanks in advance.