DAPI, TxRED and FITC

Hi,

Could anyone help me find a pipeline that can measure the fluorescence intensity of each of the following pictures?
Thank you,
DAPI:


TxRED:

FITC:

Welcome!

Are you looking for per-cell measurements, or the overall intensity in the entire image?

Note: you have pretty severe field-of-view illumination artifacts so you definitely will want to correct the illumination before taking measurements. You might want to read more about those modules to see the options.

Dear Anne,

I have changed the pictures above to better ones. I would like to look at the overall intensity of FITC vs. TxRed.

Thank you.

Great! I would suggest you make a pipeline that just has a single analysis module: MeasureImageIntensity. Drag your images into CellProfiler, assign the names of the channels appropriately, then add the MeasureImageIntensity module. Give that a try!