I am totally new in image analysis and I got few recommendations regarding qupath software for IHC analysis.
I have to quantify CD4, CD8, CD3, CD68 and CD163 positive cells in 20 tumour slides in stromal and tumoral compartments.
I was thinking to take several pictures of each tumor slide for each marker and quantify DAB positive cells in both compartments that I would have previously defined by training (machine learning based method.
As I have only one week for performing those analyses (short time!), I’ve prefered asking qupath community for getting the different steps I should do to get those cell quantifications.
I’ve attached 2 pictures of 2 tumors slides stained with CD163 antibody. Sorry for my basic questions,
I am pretty sure many people did this job! Many thanks for your help