Hi there @petebankhead,
I’m trying to detect DAB in the cytoplasm, and I watched your videos on how to do that. Now I made a semi-optimized workflow. The only problem is that I have different cell types in my tissue cores, like some giant cells and some smaller ones, including red blood cells and white blood cells. I cannot use the same cell expansion for them; otherwise, it considers red blood cells as giant cells. I can ignore them as I mainly work with tumor cells, but is there any way to apply an automatic cell expansion based on the cell size? So that I can finally classify the expression of the protein in each cell type. As I have different tissue cores in one slide, I guess I cannot apply the detection classifier?
My next question is when I select the tissue microarrays and arrange them in rows and columns, I cannot draw on the tissue or use the wand tool anymore. So I guess the only way is first to classify them and then select the TMAs, am I right?