I have stained my tissue sections using IHC and the stains are Hematoxylin and DAB. I try to detect the positivity (DAB) and measure the intensity of the area. But in my case, the positivity is mainly in the cytoplasm as the protein I am studying has mitochondrial origin. So when I use positive cell detection it gives me a percentage that does not make sense.
I’m wondering how I can detect DAB positivity correctly and measure the Intensity of the positive area. If you think there’s also other software than Qupath that I can benefit from better, please let me know. If you guide me through this I will appreciate it.