Hey ImageSC Community,
I am building a pipeline of sorts for analyzing whole slide histology images in QuPath but I am attempting to quantify the alignment of tendon/collagen as well. For this I have turned to CurveAlign but have found little resources to discus how to best go about using that software.
That being said I figured I would reach out here to see if anyone has used the software in the past and could supply any insight into the best practices for it.
At the moment, I have been using smaller regions of interest rather than the WSI. This ROI is about 3100 x 2144 pixels and looks like this:
I am running it at the 0.1 in the primary parameters as I have seen the best results from that so far. I haven’t attempted to use CT-FIRE yet but will look into it soon.
Overall I think CurveAlign does a fairly decent job quantifying alignment but I ran a test image of just cells and it returned a 20% alignment value which, in theory, should have 0 alignment as there was no tendon or collagen present.
Here is the link to CurveAlign’s website:
Here is what the tool looks like:
As always, Thank you for any insights!