I’m looking to create some ground truth images to help validate my pipelines. I’m wondering the best way to do it. I have tried CellProfiler IdentifyObjectsManually module, but it’s a little cumbersome. I also see this method here:
Not tried that yet though. Does anyone have any suggestions of ways they like to do this? I have a 2D co-culture system of two different cell types, and a couple of channels per image. Any recommendations on the number of cells to annotate/number of images to annotate would also be helpful!