I am trying to create a binary image from an SEM image with a result similar to that of this image:
This is an example of some of the images I would like to perform this operation so that I can later measure properties like size distribution of the white regions separated by the black borders:
A tip 5000x spot 4 50 us.tif (951.8 KB)
Above is the original image, but I will post a png copy so that it shows on the forum:
I have tried to use a gaussian filter with a sigma of two to smooth out the surface, then making that image a binary with this result that is much different than the above image from literature:
I also tried to subtract an image with a much larger gaussian filter (sigma = 50) before smoothing with a smaller gaussian, to try to even out any brightness inconsistencies, but the result is still different from the example:
Does anybody have any suggestions for a different way of doing this?