Sample image and/or macro code
I used a polygon in ImageJ to select the area of interest (a bat wing) and now I want to move the image over to cell profiler for analysis.
I am trying to get a mask of the wing area so I can identify fluorescent areas on the wing. I prefer to use CellProfiler for this because I already have a pipeline to use for the future analysis.
Do I use identify primary object? What parameters do I set so I get one object (the wing)?
When I use the identify primary object it breaks down the wing into 5 or 6 objects instead of the one (wing) object.