I am new to using CellProfiler and needed some help designing a module to assess the nuclear pH2AX stain in cells from images taken at 20X.
So far I’ve designed a pipeline that looks like this:
Colortogray (both nuclei (blue) and pH2AX (red))
Identify Primary Objects, nuclei
Identify Primary Objects, pH2AX
MeasureObjectIntensity, measure pH2AX from pH2AXimage
Relate Objects, Input child object= pH2AX, Input parent objects=Nuclei and check YES to calculate per parent means for all child measurements
Display data online, display pH2AX mean instensity
However, I am unable to see the measurement for per parent mean of child measurements.
Where does this show up?
Also, is there a better pipeline that I could use to assess a nuclear stain using dapi for nuclei parent reference?
I’ve attached example images for reference.
Thanks for your help.