Good day. I’m currently creating a heat map from a stack of images to show the area where a particular lesion is most found. I did this through Z-Project, particularly Sum Slice, LUT, and creation of a Calibration Bar adjusted to 0-100.
However, this only measures the intensity/value of a signal. Is there a way for me to make the calibration values into the frequency of the signal/how often is the signal found across all the images in the stack? Thank you very much.