Here is the macro:
//setThreshold(0, 100); setOption("BlackBackground", true); run("Convert to Mask", "method=Default background=Light black"); run("Fill Holes", "stack"); run("Tiff...");
Pretty straightforward, all of my images work well with a threshold at value 100 and I just want to make them binary and open the save dialogue so I can type a new name for the Binary versions.
However, when I run the macro, it never actually uses threshold 100. It seems to consistently use a higher one, resulting in a ton of artifacts and false positives.
Any ideas how to fix this? I was hoping this would be a very simple macro to make