Hi, I’m trying to create an automated method to count cellular inclusion bodies that are “around” a nuclei, i.e. a particle.
I haven’t figured out a way to do this in ImageJ/FIJI.
Conceptually, I have a stack (image1, image2).
What I’d like to do is use “image1” to define the particle (in this case nuclei) and then build a radius (for example, 200px) ROI around this particle.
I would pass this ROI to “image2” which would then count the particles (inclusion bodies) in the ROI.
There would be multiple ROIs per image, so ideally I would like to get multiple readings of the particles (inclusion bodies) per cell in each image.
I can do most of the macro programming, but I’m stuck on figuring out how best to create the radius ROI. Using something like the watershed analysis (which finds the center) should be useful, but I can’t figure out how to proceed.
Thanks for any tips or help!