Create a average grey value tif out of many tifs

For a project I have a few hundred Images (TIF 16bit) with the same parts on it, only varying by their Grey value.
Is there a program where I can put in all the 16bit Tifs and get one average 16bit Tif in the end?
Because in the end I want to measure the average Grey value (on specific lines) by using ImageJ

I hope you understand what I mean and can help me :slight_smile:


You can open all your images in a stack using

File > Import > Image Sequence…

Then use Image > Stacks > Z Project… to make an Average Projection of all your images.


Cool that´s exactly what I was searching for! Thx