I made a pipeline using cellprofiler in which I do cell segmentation on ependymal tissue.
Inside the cells there are two types of objects that can be found centrosome and centrioles.
I used the relateObject module in my pipeline to count the centriole and centrosome in each cell.
I now want to use that in CPA to classify the cell in 4 stages. I want to make rules like:
IF(Cells_Children_centrioles_Count = X then it goes in one class)
I also want to combine rules, so for example to be in stage 1 the cell has to have 1 or 2 centrosomes and 1 or 2 centriole and the centriole has to be small.
But I do not really understand how to modify the rules and how to create new ones and how it split all the objects in categories.
Can someone help me with that?