Counting the total number of cells in 6 well plate


I am trying to do find the total cell count in 6 well plate format. I have 4x images of the entire wells (stitched) and the cells are unstained.
I tried different thresholding methods (Manual and auto) followed by “analyze particles” option, but they don’t seem to be so accurate. Is there any other method that I can follow?

Any suggestions would be well appreciated.

Thank you.


Can you share an ORIGINAL image file with us here? This is the best way for us to help you… you can check this older post - it might be most relevant to your situation: