Counting the total number of cells in 6 well plate

Hi,

I am trying to do find the total cell count in 6 well plate format. I have 4x images of the entire wells (stitched) and the cells are unstained.
I tried different thresholding methods (Manual and auto) followed by “analyze particles” option, but they don’t seem to be so accurate. Is there any other method that I can follow?

Any suggestions would be well appreciated.

Thank you.

@GKG

Can you share an ORIGINAL image file with us here? This is the best way for us to help you… you can check this older post - it might be most relevant to your situation: