I was wondering if anyone knew how to count the number of ROIs within an are specified by a large ROI overlay?
I wrote this piece of code that will take a predrawn large ROI that covers a certain area, for example, 50/3000 smaller ROIs, and clears the outside of all ROIs that are outside of the overlay. Any ideas on how I can count the number of mini ROIs within the larger overlay ROI? I tried “analyze particles”, but the image needs to be thresholded/8-bit.
Thanks for the help!